Nevertheless, by 1905, a report on the seaweed industries in Japan noted the “very important use [of pure-grade agar] as a culture medium in bacteriological work.” It’s safe to say that, around the turn of the 20th century, agar had moved from an inconspicuous kitchen jelly to an indispensable scientific substance.
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This anomaly is indicative of the larger challenge of culturing various microbial species, referred to as microbial “unculturability.” This cannot be explained by the use of agar alone or by the substitution of an alternative gelling agent, but rather by the difficulties in consistently recreating on an agar plate the multi-variable environment in which microbes grow naturally. Given such challenges, the risk of shortages, and the vulnerabilities of the agar supply chain, why is it so difficult to find suitable alternatives?